TSS Buffer
Based on the Bennett lab TSS protocol
Pre-sterilized components
Makes 50mL TSS Buffer
| Component | Stock Concentration | Sterilization method | Amount |
|---|---|---|---|
| LB Media | 1x | Autoclaved | 42.5mL |
| PEG-3350 | 1g/mL | Filter-sterilized | 5mL |
| MgCl2 | 1M | Autoclaved | 1mL |
| DMSO | 100% | – | 2.5mL |
Store at 4°C.
Post-sterilized components
Makes 50mL TSS Buffer
| Component | Stock Concentration | Amount |
|---|---|---|
| PEG-3350 | pure | 5g |
| MgCl2 | 1M | 1mL |
| DMSO | 100% | 2.5mL |
| LB media | 1x | make up to 50mL (~46.5mL) |
Filter sterilize and store at 4°C.